法医学ジャーナル

Researchers were driven by the urge to explore the fundamental physics behind a perplexing forensic enigma. Their findings, featured in the journal "Physics of Fluids," reveal theoretical insights into the connection between the advancing vortex ring generated by the discharge of firearms and the subsequent blood backflow. Prior to this, the research group had established an all-encompassing analytical framework for these highly concentrated, self-similar vortex rings, which exhibits a mathematical linkage to the theory of quantum oscillators.

In an effort to enhance the monitoring of persistent drug use in individuals, a group of experts hailing from the National University of Singapore (NUS) has introduced an innovative approach. Led by a professor from the NUS Department of Pharmacy, the team has uncovered three new urine biomarkers. These biomarkers could be employed to detect the consumption of the emerging synthetic cannabinoid ADB-BUTINACA, classified as a novel psychoactive substance (NPS). The same cutting-edge technology also enables the differentiation between natural and synthetic cannabinoids.

Legal researchers may continue to face limitations in the conventional sample preparation methods for analysis, such as those involving blood or urine. They are actively exploring advanced methodologies, especially as modern instrumentation like gas and liquid chromatographs paired with mass spectrometers becomes more agile and swift in response.

Playing a pivotal role in transcription and replication control, developmental reprogramming, retroelement silencing, and various other genomic activities, DNA methylation is an essential epigenetic regulatory mechanism. In the context of mammalian development, the establishment of a specific DNA methylation pattern in germ cells is a prerequisite for embryonic development. However, our understanding of DNA methylation in germ cells of other animals remains limited. To address this gap in knowledge, we conducted a comprehensive analysis of the single-cell methylome of chicken diplotene oocytes. Through the development of a methylation-based segmentation approach for the chicken genome, we identified methylated gene promoters exclusive to oocytes. This extensive characterization of methylation patterns in these cells revealed that they closely mirror the chromosomal distribution observed in somatic tissues, despite the presence of a distinct transcriptionally hyperactive genome architecture in chicken diplotene oocytes. Our findings shed light on the intricate role of DNA methylation in these cells.

We employed Illumina Hiseq 2500 technology to conduct chloroplast genome sequencing for Salvia bowleyana, S. splendens, and S. officinalis. This endeavor aimed to elucidate their evolutionary relationships comprehensively and establish molecular markers for species identification. The chloroplast genomes of these species exhibited lengths of 151,387, 150,604, and 151,163 base pairs, respectively. Within these genomes, the inverted repeat (IR) regions harbored six genes: ndhB, rpl2, rpl23, rps7, rps12, and ycf2. In terms of repeat structures, S. bowleyana, S. splendens, and S. officinalis showcased 29 tandem repeats, 35 simple sequence repeats, and 24 simple sequence repeats, along with 47, 49, and 40 interspersed repeats, respectively. Notably, three distinct intergenic sequences (IGS)-rps16-trnQ-UUG, trnL-UAA-trnF-GAA, and trnMCAU- atpE-enabled the differentiation of the 23 Salvia species. Genetic distance analysis identified a total of 91 intergenic spacer sequences, with the ycf3-trnS-GGA and trnG-GCC-trnM-CAU regions demonstrating the highest K2p values among the three Salvia species under investigation. Furthermore, our phylogenetic analysis revealed that the 23 Salvia species formed a monophyletic group. This study also unveiled the development of two sets of genus-specific DNA barcode primers. These findings serve as a robust foundation for understanding the phylogenetic classification of the three Salvia species. Additionally, the distinctive intergenic regions hold the potential to distinguish Salvia species based on both phenotypic traits and gene segment differentiation.

Various techniques including AFM, ellipsometry, surface tensiometry, surface dilational rheology, and infrared reflection-absorption spectroscopy (IRRAS) were employed to probe the interactions between DNA and lysozyme within the surface layer. In the experiments, a concentrated DNA solution was introduced into an aqueous subphase beneath a dispersed lysozyme layer. Notably, when compared to DNA's interactions with a monolayer of a cationic synthetic polyelectrolyte, the optical properties of the surface layer exhibited rapid changes following DNA injection, while the dynamic dilational surface elasticity remained relatively stable. This observation suggests the absence of a continuous network of DNA/lysozyme complexes. The swift increase in optical signals following DNA injection behind a lysozyme layer implies that DNA penetration is predominantly governed by diffusion. Furthermore, AFM images vividly illustrate the formation of elongated strands within the surface layer under low surface pressures. In contrast, increased surface compression leads to the emergence of folds and ridges, rather than the formation of a network of DNA/lysozyme aggregates. These results suggest that weaker interactions between lysozyme and duplex DNA, along with the stabilization of unpaired nucleotide loops at high local lysozyme concentrations in the surface layer, contribute to the generation of more disordered aggregates.

Chronic liver rejection poses a complex challenge in transplantation, as some patients do not respond to increased immunosuppression. Killer cell immunoglobulin-like receptors and their interactions with Class I Human Leukocyte Antigens (HLA-I) play a crucial role in predicting Natural Killer (NK) cell alloreactivity and influencing acute liver graft rejection. However, their relevance in CR remains a subject of debate. In this study, we investigated KIR and HLA genotypes in 513 liver transplant recipients using sequence-specific oligonucleotide (PCR-SSO) methods. We examined KIRs, human leukocyte antigen C (HLA-C) genotypes, KIR gene combinations, and KIR/HLA ligand interactions in the entire cohort and compared them between CR (n=35) and no-ongoing rejection (NCR=478) cases. It was found that the presence of activating KIR (aKIR) genes in recipients (rKIR2DS2+ and rKIR2DS3+) increased the risk of CR compared to the NCR group (p=0.013 and p=0.038). Inhibitory KIR (iKIR) genes in recipients, particularly rKIR2DL2+, significantly elevated the CR rate compared to their absence (9.1% versus 3.7%, p=0.020), and KIR2DL3 also had a significant impact on increasing CR (13.1% versus 5.2%; p=0.008), with no effect on NCR. Furthermore, CR was observed in cases with HLA-I mismatches (MM), and the absence of the donor (d) HLA-C2 ligand (dC2−) increased the risk of CR compared to its presence (13.1% versus 5.6%; p=0.018). A significant increase in CR was noted in cases with rKIR2DL3+/dC1− (p=0.015), rKIR2DS4/dC1− (p=0.014), and rKIR2DL3+/ rKIR2DS4+/dC1− (p=0.006) combinations. Long-term patient survival was significantly lower in recipients with rKIR2DS1+rKIR2DS4+/dC1− at 5-10 years post-transplant. This study highlights the influence of rKIR/dHLA-C combinations and aKIR gene variations in increasing the risk of CR, as well as the impact of KIR2DS1+/C1-ligands and KIR2DS4+/C1-ligands on long-term graft survival.

The purpose of this study has a dual focus: firstly, to establish correlations between the characteristics of trabecular bone microstructure within different boundaries and an individual's chronological age and gender, thus facilitating the assessment of potential age and gender-related changes in TBM boundaries within the mandible. Secondly, the study aims to evaluate how these trabecular microstructural boundaries correspond to categorized age groups. A total of twenty cone-beam computed tomography scans were retrospectively selected from an adult patient dataset, spanning ages from 22 to 43 years. The region of interest in the mandible encompassed the interdental space between the second mandibular premolar and the first mandibular molar, along with the trabecular space beneath and between the apices. The DICOM images from the CBCT scans were pre-processed, transformed, segmented using an intelligent semi-automated thresholding technique, and quantified. Furthermore, TBM characteristics were derived, and a statistical analysis was conducted using a two-tailed Pearson correlation test.

This study delves into the ever-evolving landscape of novel psychoactive compounds, exploring emerging trends in their synthesis, distribution, and consumption. We analyze the dynamic interplay between regulatory efforts and the clandestine innovation of new substances, shedding light on the challenges posed to public health and law enforcement. By examining the latest developments in the field, this research aims to provide valuable insights for policymakers, clinicians, and researchers to address the complex issues associated with novel psychoactive compounds effectively.

Forensic researchers face substantial hurdles when it comes to retrieving fingerprint evidence from gunshot casings. At crime scenes, both fired and unfired casings are commonly found, yet the recovery of fingerprints and the establishment of a connection between the shooter and the firearm remain persistently challenging tasks. This difficulty arises from the extreme conditions that projectile casings undergo during firing, as well as the methodologies employed for the creation and detection of fingermarks.