代替医療と統合医療

Metabolomics Study of Protective Effects of Shexiang Baoxin Pill and its Bioactive Constitutes Combination in Treating the Early Period of Acute Myocardial Infarction in Rats

Abstract

Xiang L, Han L, Jiang P, Wang S, Hu Y, Zhang W and Liu R

Ginsenoside Re, ginsenoside Rb1, muscone, cinnamic acid, bufalin, cholic acid and borneol are the main active ingredients of Shexiang Baoxin Pill (SBP) for protective effects on acute myocardial infarction (AMI). In this study, the protective effects of SBP, single component treatments and combination treatment of 7 bioactive ingredients of SBP (SFSBP) were investigated by metabolomics technology. Liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) has been used to analyze the plasma samples of AMI rats. Partial least squares discriminate analysis (PLS-DA) models were built to evaluate the protective effects of different treatments at whole level. Reverse effect on mean levels of 19 identified biomarkers were also carried out to investigate the protective effects of different treatments. The results showed that the protective effect of multi-component medicines (SBP and SFSBP) was superior to 7 single-component treatments, demonstrated the multi-effective advantages of multicomponent treatments (SBP and SFSBP). Meanwhile, the reverse effects of SFSBP were inferior to SBP, indicating that there might be other bioactive components in SBP taking responsibility for the protection effect on AMI rats, and the simplified formula of SBP should be further optimized, while also revealed the rationality of TCM formula, and it cannot be simply substituted by a combination of bioactive ingredients.