分子遺伝医学

Design of Plasmalemma and Nucleolemma Specific Quantum Dots Stem Cell Labeling

Abstract

Wang L, Li Z, Xu MM, Sun MT, Zhou X, Ma J, Isaacs WB and Xu X

To date, there are no efficient treatments for most of human congenital, developmental, or degenerative diseases because functional cells failed to regenerate new cells following damages to original cells. Stem Cell (SC) based therapeutic approach holds tremendous promise for treating these abnormalities by replacing the damaged cells. This approach has sparked unprecedented attention in this field. However, SC researchers should adopt more robust techniques that incorporate marker genes that render stem cells visible using fluorescence and conventional microscopy. We design a unique approach to label embryonic and adult stem cells, the peptide-mediated quantum dots (QDs) SCs labeling with plasmalemma and nucleolemma specific fusion peptides. Therefore, the targeted SCs will be distinguishable by the new markers to report grafted cells from endogenous cardiomyocytes and to display the trans-differentiation from the SCs. The fluorescence strength of nanocrystal QDs will surmount the interference of the false signals originating from auto-fluorescence of cardiac tissues with their features for anti-bleaching of nonorganic dye.