法医学ジャーナル

Objective: The Autonomic Nervous System (ANS) has been associated with numerous atherosclerosis-induced
cardiovascular events, such as myocardial infarction and aortic disease. Although evidence suggests a relationship
between autonomic dysfunction and atherosclerotic disease, the underlying mechanisms are still under
investigation. The purpose of this study is to investigate the effect of ANS to the development of atherosclerosis and
vice versa, in human thoracic aorta.
Methods: An autopsy analysis from three segments of the thoracic aorta was performed; ascending aorta, aortic
arch, descending aorta, using 52 unselected adult cadavers (38 male, 14 female-mean age 64.4 years; age range
19-90 years). Subjects were divided in two age groups (<65 years–n=26, >65 years-n=26). Tissue specimens were
macroscopically examined and histopathologically divided into 7 grades of scoring for atherosclerosis (ATHERO,
from 0=intact, to 6=thrombi formation). The relationship between ANS and atherosclerosis was depicted by further
immunohistochemical analysis for detection of neuron terminals onto the aortic wall. All data were evaluated
according to the subject’s demographic and clinical characteristics.
Results: Total 96.2% of all subjects had atherosclerosis of variable degree in one or more segments. No
aneurismal change was found. The presence of atheromas were common in all subjects regardless of age and
segment, with atherosclerosis increasing by age; Ascending aorta (r=.571, p<.001), Aortic arch (r=.655, p<.001),
Descending aorta (r=.659, p<.001). Hypertension was a significant factor in the development of atherosclerosis in
adults>65 years (r=.450, p=.023). In addition, a positive history of hypertension was statistically significant regarding
both the presence of atherosclerosis and neuron terminals in all three aortic wall segments; Ascending aorta (p=.
037), Aortic arch (p=.046), Descending aorta (p=.045). Furthermore, there was a strong negative correlation
between the ATHERO score and the presence of neuron terminals in all three aortic segments; Ascending aorta
(r=-.264, p=.041), Aortic arch (r=-.400, p=.003), Descending aorta (r=-.234, p=.047).
Conclusion: Human cadaveric studies are extremely useful in understanding the pathophysiology of ANS, along
with clinical and animal studies that are most commonly performed. These data suggest that there is a link between
autonomic disfunction and the presence of atherosclerosis in human thoracic aorta, especially when hypertension is
present. It is therefore possible that stress-induced hypertension can be considered as a potential risk factor for the
development of atherosclerosis.

The aim of this study is to explore the differentiation capacity of 17 Y-Chromosomal Short Tandem Repeat markers (Y-STRs) and to enrich the forensic Y-chromosome databases southern districts of Jordan data for a better knowledge of the frequency and distribution of Y-chromosome markers across these populations. 17 Y-Chromosome Short Tandem Repeats loci (DYS456, DYS389i, DYS390, DYS389ii, DYS458, DYS19, DYS385a, DYS385b, DYS393, DYS391, DYS439, DYS635, DYS392, TAGA H4, DYS437, DYS438, and DYS448) were analyzed in blood samples of 160 unrelated males which were collected freshly and randomly from the governmental hospitals from the Southern Districts of Jordan (Ma’an, Karak, Tafila, Aqaba). The frequency of the alleles among Southern Districts of Jordan ranged from 0.61 to 89.6. The genetic diversity values for each Y-chromosome STRs ranged from a minimum of 0.2 to a maximum of 0.8. The minimal haplotype (calculated manually ≥ 75%) is composed of four STRs which were observed in the Southern Districts samples equivalent to most commonly loci which would be appear at this order, DYS392, DYS437, DYS438 and DYS439, ranged from a minimum of 75.5 % for (DYS439), 82.4% for (DYS438), 87.5 % for (DYS437), 89.7% for (DYS392), with mean value equal 83.8%. The similarity of our result to other Districts of Jordan is in the following order: Ajloun (100%), Irbid (75%), Jarash (25%), Mafraq (75%), Amman (75%), Zarqa (50%), Madaba (50%), and Salt (50%). The similarity of our result to other regional countries is in the following order: (Saudi Arabia (75%), Iraq (75%), Qatar (100%), Kuwait (25%), and United Arab Emirates (50%) Bahrain (50%), Oman (100%) and Yemen (75%), Syria (50%), Lebanon (0%), Libya (75%), Egypt (75%), Mauritania (75%), Tunis (75%), Algeria (75%) Morocco (100 %), Sudan (100%), Somali (25%). Our data shows that all Y –STR’s loci typed were a bimodal distribution in paternity testing and in individual identification cases. These analyses support use of the haplotype population data for estimating Y-STR profile frequencies for populations residing in South Jordan and provides an informative analysis of the Y-Chromosomal STR diversity in the Arab population and emphasizes the discrimination capacity of high-resolution Y-STR typing that can be achieved in the Arab population for the forensic DNAcasework.