応用および計算数学ジャーナル

Using Mathematics to Estimate the Affinity of Aptamer Target Binding

Abstract

Shaik Yusuf

In the intricate realm of molecular interactions, the utilization of mathematics plays a pivotal role in deciphering and predicting the affinity of aptamer-target binding. Aptamers, short single-stranded nucleic acids or peptides, have emerged as versatile molecular recognition elements, showcasing an inherent ability to selectively bind to specific target molecules with remarkable affinity. The estimation of this binding affinity is a complex task that necessitates a sophisticated mathematical framework. Central to this estimation is the application of thermodynamics, where mathematical models are employed to unravel the energetic landscape of aptamer-target interactions. Thermodynamic parameters such as Gibbs free energy, enthalpy, and entropy are harnessed to quantify the driving forces behind binding events. The affinity constant, often represented by Kd, serves as a quantitative measure of the strength of the aptamer-target association and is a cornerstone in this mathematical exploration.