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Effects of Quercetin Induced Cell Death on a Novel Gene "URG4/URGCP" Expression in Leukemia Cells

Abstract

Yavuz Dodurga, Cigir Biray Avci, Lale Satiroglu-Tufan N, Sunde Yilmaz Susluer, Ozlem Dogan Sigva Z, Guray Saydam and Cumhur Gunduz

The present study aimed to investigate anti-proliferative and apoptotic effects of quercetin on human leukemia cells and effects of quercetin-induced cell death on a novel gene Up-regulated gene 4/upregulator of cell proliferation ( URG4/URGCP ), in leukemia cells. URG4/URGCP expression is determined by using RT-PCR. IC 50 of quercetin was determined as 25 microM in CCRF-CEM, HL–60 and K562 cells. In IC 50 dose group, URG4/URGCP expression was decreased 99% in HL-60 cells, 90% in CCRF-CEM cells, and 52% (24 hour) - 99% (72 hour) in K-562 cells. URG4/URGCP may play important roles in the development of leukemia, and might be a useful molecular marker for predicting the prognosis of leukemia via quercetin treatment.