獣医科学と技術

Comparison between Rose Bengal Plat Test and Competitive Enzyme Linked Immunosorbent Assay to Detect Bovine Brucellosis in Kigali City, Rwanda

Abstract

Rosine Manishimwe, Julius Ntaganda, Richard Habimana, Kizito Nishimwe, Maurice Byukusenge, Fausta Dutuze, Jean de Dieu Ayabagabo, Umurerwa Lydia, Jean Claude Rukundo

Bovine brucellosis is a zoonosis known to be a major public health hazard of great economic importance worldwide. Its detection in cattle is frequently done using serological tests like Rose Bengal Plate Test (RBPT) and Competitive- Enzyme Linked Immuno Sorbent Assay (C-ELISA). Few studies have been conducted to compare the serological tests most commonly used to detect bovine brucellosis in Rwanda.

The study was conducted in Kigali city, the capital of Rwanda, to compare RBPT and C-ELISA test in detection of bovine brucellosis. Two thousands and seventeen (2017) cattle serum samples were collected, from 157 randomly selected farms, and were tested for bovine brucellosis using RBPT and C-ELISA.

The overall prevalence given by RBPT (2.03%) was greater than the prevalence given by C-ELISA (1.7%). The study revealed that even if the observed difference between prevalence given by RBPT and the prevalence given by C-ELISA was significant (p-value<0.05), the agreement between the two tests has been found excellent with a Kappa of 0.92.

The study has provided information on the agreement of the two serological tests commonly used to detect bovine brucellosis in Kigali. It is known that the agreement between two tests varies depending on the prevalence, thus there is a need for the study to be extended to the other provinces of Rwanda where the prevalence is probably different to the prevalence in Kigali.